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PUBLICATION list

Please visit the IST Research Explorer to find all publications of Siegert group members

2021

Alessandro Venturino, Rouven Schulz, Héctor De Jesús-Cortés, Margaret E. Maes, Bálint Nagy, Francis Reilly-Andújar, Gloria Colombo, Ryan John A. Cubero, Florianne E. Schoot Uiterkamp, Mark F. Bear, Sandra Siegert.
Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain

Accepted in Cell Reports

Margaret E Maes, Gabriele M Wögenstein, Gloria Colombo, Raquel Casado-Polanco, Sandra Siegert

Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment

bioRxiv: https://www.biorxiv.org/content/10.1101/2021.06.02.442814v1

https://doi.org/10.1101/2021.06.02.442814

Schulz R, Korkut-Demirbaş M, Colombo G, Siegert S, “Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses, revision (2021)

https://www.biorxiv.org/content/10.1101/2021.06.21.449162v1

We developed chemical-inducible GPCRs based on DREADD allowing CNO-mediated stimulation and recapitulated entire signaling of β2-adrenergic receptor including also the inflammatory and migratory response in microglia.

Venturino, A, Siegert S,

Minimally-invasive methods and quantification for microglia-mediated perineuronal net disassembly”, STAR Protocols, 2021; accepted (2021)

Maes ME, Wögenstein GM, Colombo G, Casado-Polanco R, Siegert S, “Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment”, Mol Ther Methods Clin Dev, 2021; 23:210-224

https://10.1016/j.omtm.2021.09.006

We optimized adeno-associated viruses (AAVs) transduction efficiency and specificity for retinal microglia in vivo and found that certain disease environments influence microglial susceptibility to viral transduction

Venturino A, Schulz R, de Jesus-Cortex H, Reilly-Andujar F, Maes ME, Nagy B, Cubero RJA, Colombo G, Schoot Uiterkamp FE, Bear M, Siegert S, “Microglia dismantle mature perineuronal nets upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain”, Cell Reports, 2021, in press,

https://doi.org/10.1016/j.celrep.2021.109313

We found that repeated anesthetic dosage of ketamine induces microglia-dependent PNN removal, which reinstates juvenile-like plasticity. We also recapitulated the ketamine-mediated effects with selective 60-Hz light entrainment paradigm.

Gloria Colombo, Ryan John Abat Cubero, Lida Kanari, Alessandro Venturino, Rouven Schulz, Martina Scolamiero, Jens Agerberg, Hansruedi Mathys, Li-Huei Tsai, Wojciech Chachólski, Kathryn Hess, Sandra Siegert

Microglial MorphOMICs unravel region- and sex-dependent morphological phenotypes from postnatal development to degeneration

https://www.biorxiv.org/content/10.1101/2021.11.30.470610v1

2019

M.E. Maes, G. Colombo, R. Schulz, S. Siegert
Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges
Neuroscience Letters 707 (2019), doi: 10.1016/j.neulet.2019.134310

2016

Tsai L-H, Siegert S
“How microRNAs are involved in splitting the mind”
JAMA Psychiatry 2016; 73(4): 409-10, doi: 10.1001/jamapsychiatry.2015.3144

2015

Mungenast AE*, Siegert S*$, Tsai L-H (*contributed equally, $corresponding author)
“Modeling Alzheimer’s disease with human induced pluripotent stem (iPS) cells”
Molecular and Cellular neuroscience 2015; 73:13-31, doi: 10.1016/j.mcn.2015.11.010

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Siegert S, Seo J, Kwon EJ, Rudenko A, Cho S, Wang W, Flood Z, Martorell AJ, Ericsson M, Mungenast AE, Tsai L-H
“The schizophrenia risk gene miR-137 alters presynaptic plasticity”
Nature Neuroscience 2015; 18(7): 1008-16, doi: 10.1038/nn.4023

Comment in:

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Rei D, Mason X, Gräff J, Seo J, Rudenko A, Wang J, Rueda R, Siegert S, Cho S, Canter RG, Mungenast A, Deisseroth K, Tsai L-H
“The BLA bidirectionally modulates stress-induced hippocampal learning and memory deficits through a p25/Cdk5-dependent pathway”
Proc Natl Acad Sci USA 2015; 112(23): 7291-6, doi: 10.1073/pnas.1415845112

2012

Siegert S, Cabuy E, Gross Scherf B, Kohler H, Panda S, Le, Y-Z, Fehling HJ, Gaidatzis D, Stadler M, Roska B
“Transcription factor code and disease map for retinal cell types”
Nature Neuroscience 2012; 15(3): 487-95, doi: 10.1038/nn.3032 Awarded the SWISS OphthAWARD in the category “Best experimental work”

2010

Busskamp V, Duebel J, Balya D, Fradot M, Viney TJ, Siegert S, Groner AC, Cabuy E, Forster V, Seeliger M, Biel M, Humphries P, Paques M, Mohand-Said S, Trono D, Deisseroth K, Sahel JA, Picaud S, Roska B
“Genetic reactivation of cone photoreceptors restores complex visual responses in Retinitis pigmentosa”
Science 2010; 329(5990): 413-7, doi: 10.1126/science.1190897

Comment in:

Featured on Science cover (July 2010, Vol 329, No 5990)

2009

Siegert S, Gross Scherf B, Del Punta K, Didkovsky N, Heintz N, Roska B
“Genetic address book for retinal cell types”
Nature Neuroscience 2009; 12(9): 1197-204, doi: 10.1038/nn.2370

Featured on the Nature Neuroscience cover (September 2009, Vol 12, No 9)

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Münch TA, da Silveira RA, Siegert S, Viney TJ, Awatramani GB, Roska B
“Approach sensitivity in the retina processed by a multifunctional neuronal circuit”
Nature Neuroscience 2009 Oct; 12(10): 1308-16, doi: 10.1038/nn.2389

Comment in: “A night vision neuron gets a day job” by Oesch N, Diamond J [Nat. Neurosci. 2009]

2007

Viney TJ, Balint K, Hillier D, Siegert S, Boldogkoi Z, Enquist LW, Meister M, Cepko CL Roska B
Local retinal circuits of melanopsin-containing ganglion cells identified by transsynaptic viral tracing
Current Biology 2007 Jun 5; 17(11): 981-8

 Comment in:

2006

Siegert S, Schnierle P, Schnierle BS
Novel anti-viral therapy: Drugs that blocks HIV entry at different target sites
Mini Reviews in Medicinal Chemistry 2006 May; 6(5): 557-62

2005

Siegert S, Thaler S, Wagner R, Schnierle BS
Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
AIDS Research and Therapy 2005 Sep 12, 2:7